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connect oligoperfect tool  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher connect oligoperfect tool
    Connect Oligoperfect Tool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/connect+oligoperfect+tool/bio_rxiv__2024__11__08__622637-405-27-30
    Average 90 stars, based on 1 article reviews
    connect oligoperfect tool - by Bioz Stars, 2026-09
    90/100 stars

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    Fluorescence In Situ Hybridization:

    Article Title: Cytoplasmic lncRNAs nucleate signalling pathways to define metastable state dynamics and determine phenotypic output
    Article Snippet: Data analysis was conducted using Real-Time qPCR Connect Data Analysis Tool (Thermo Fisher Scientific Connect Platform). .. FISH probe amplicon regions between 260-300bp were determined based on transcript sequences (Ensembl release 105 ) and primers to amplify these regions were then designed using the Connect OligoPerfect tool (Thermo Fisher). .. RNA purified from cells known to express target RNAs at high level was reverse transcribed with QuantiTect reverse transcription kit (Qiagen, cat. 205311) using oligo-dT primers following the manufacturer’s recommendations. cDNA was then amplified by the Expand high fidelity PCR system (Roche, cat. 11732641001) using specific primers and PCR-products were then resolved by agarose gel electrophoresis.

    Amplification:

    Article Title: Cytoplasmic lncRNAs nucleate signalling pathways to define metastable state dynamics and determine phenotypic output
    Article Snippet: Data analysis was conducted using Real-Time qPCR Connect Data Analysis Tool (Thermo Fisher Scientific Connect Platform). .. FISH probe amplicon regions between 260-300bp were determined based on transcript sequences (Ensembl release 105 ) and primers to amplify these regions were then designed using the Connect OligoPerfect tool (Thermo Fisher). .. RNA purified from cells known to express target RNAs at high level was reverse transcribed with QuantiTect reverse transcription kit (Qiagen, cat. 205311) using oligo-dT primers following the manufacturer’s recommendations. cDNA was then amplified by the Expand high fidelity PCR system (Roche, cat. 11732641001) using specific primers and PCR-products were then resolved by agarose gel electrophoresis.



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    90
    Thermo Fisher connect oligoperfect tool
    Connect Oligoperfect Tool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/connect+oligoperfect+tool/bio_rxiv__2024__11__08__622637-405-27-30
    Average 90 stars, based on 1 article reviews
    connect oligoperfect tool - by Bioz Stars, 2026-09
    90/100 stars
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